Evaluation of fractionated circulating filarial antigen in diagnosis of bancroftian filariasis.
Identifieur interne : 00D135 ( Main/Exploration ); précédent : 00D134; suivant : 00D136Evaluation of fractionated circulating filarial antigen in diagnosis of bancroftian filariasis.
Auteurs : K. Cheirmaraj [Inde] ; K A Parkhe ; B C HarinathSource :
- The Journal of tropical medicine and hygiene [ 0022-5304 ] ; 1992.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Anticorps antihelminthe, Antigènes d'helminthe.
- diagnostic : Filariose lymphatique.
- immunologie : Filariose lymphatique, Microfilaria, Wuchereria bancrofti.
- Animaux, Fractionnement chimique, Humains, Test ELISA.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antibodies, Helminth, Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Microfilariae, Wuchereria bancrofti.
- Animals, Chemical Fractionation, Enzyme-Linked Immunosorbent Assay, Humans.
Abstract
Circulating filarial antigen (CFA) isolated from the plasma of microfilaraemic patients was fractionated on an Ultrogel ACA 34 column. The second protein peak (CFA2) showing filarial antigen was further fractionated by DEAE-cellulose column chromatography into two fractions (CFA2 DE1 and DE2). CFA2 DE1 fraction, showing antigenic activity, was further evaluated in an ELISA for its diagnostic use in bancroftian filariasis. Studies with CFA2 DE1 and anti-CFA2 DE1 antibody showed that they were highly active in the detection of filarial antibody and antigen in asymptomatic microfilaraemia sera and thus obviate the need for the tedious night blood collection and examination. Fractionated filarial plasma can be another candidate antigen for immunodiagnosis of bancroftian filariasis.
PubMed: 1740819
Affiliations:
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Le document en format XML
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Cheirmaraj</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Parkhe, K A" sort="Parkhe, K A" uniqKey="Parkhe K" first="K A" last="Parkhe">K A Parkhe</name></author><author><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1740819</idno><idno type="pmid">1740819</idno><idno type="wicri:Area/PubMed/Corpus">005979</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005979</idno><idno type="wicri:Area/PubMed/Curation">005979</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">005979</idno><idno type="wicri:Area/PubMed/Checkpoint">005979</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">005979</idno><idno type="wicri:Area/Ncbi/Merge">002652</idno><idno type="wicri:Area/Ncbi/Curation">002652</idno><idno type="wicri:Area/Ncbi/Checkpoint">002652</idno><idno type="wicri:doubleKey">0022-5304:1992:Cheirmaraj K:evaluation:of:fractionated</idno><idno type="wicri:Area/Main/Merge">00DC19</idno><idno type="wicri:Area/Main/Curation">00D135</idno><idno type="wicri:Area/Main/Exploration">00D135</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluation of fractionated circulating filarial antigen in diagnosis of bancroftian filariasis.</title><author><name sortKey="Cheirmaraj, K" sort="Cheirmaraj, K" uniqKey="Cheirmaraj K" first="K" last="Cheirmaraj">K. Cheirmaraj</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram</wicri:regionArea><wicri:noRegion>Sevagram</wicri:noRegion></affiliation></author><author><name sortKey="Parkhe, K A" sort="Parkhe, K A" uniqKey="Parkhe K" first="K A" last="Parkhe">K A Parkhe</name></author><author><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name></author></analytic><series><title level="j">The Journal of tropical medicine and hygiene</title><idno type="ISSN">0022-5304</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (analysis)</term><term>Antigens, Helminth (analysis)</term><term>Chemical Fractionation</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Microfilariae (immunology)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (analyse)</term><term>Antigènes d'helminthe (analyse)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (immunologie)</term><term>Fractionnement chimique</term><term>Humains</term><term>Microfilaria (immunologie)</term><term>Test ELISA</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Filariose lymphatique</term><term>Microfilaria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Microfilariae</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chemical Fractionation</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Fractionnement chimique</term><term>Humains</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Circulating filarial antigen (CFA) isolated from the plasma of microfilaraemic patients was fractionated on an Ultrogel ACA 34 column. The second protein peak (CFA2) showing filarial antigen was further fractionated by DEAE-cellulose column chromatography into two fractions (CFA2 DE1 and DE2). CFA2 DE1 fraction, showing antigenic activity, was further evaluated in an ELISA for its diagnostic use in bancroftian filariasis. Studies with CFA2 DE1 and anti-CFA2 DE1 antibody showed that they were highly active in the detection of filarial antibody and antigen in asymptomatic microfilaraemia sera and thus obviate the need for the tedious night blood collection and examination. Fractionated filarial plasma can be another candidate antigen for immunodiagnosis of bancroftian filariasis.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name><name sortKey="Parkhe, K A" sort="Parkhe, K A" uniqKey="Parkhe K" first="K A" last="Parkhe">K A Parkhe</name></noCountry><country name="Inde"><noRegion><name sortKey="Cheirmaraj, K" sort="Cheirmaraj, K" uniqKey="Cheirmaraj K" first="K" last="Cheirmaraj">K. Cheirmaraj</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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